拉里·达利(本·斯蒂勒 饰)生活似乎不太顺利:经常换工作,居无定所;妻子与他离婚,找了一个证券经纪人,即将再婚,自己在儿子心目中的地位也岌岌可危。 达利为了挽救自己的生活,也为了争取到儿子的抚养权,他不得不放弃“做大事”的梦想,成为了纽约自然博物馆的夜间警卫。 达利原本 以为这是一份无聊的工作,谁知道上班的第一天,不可思议的事情就发生了:博物馆里的法老金牌拥有神奇的魔法,当夜晚来临的时候,馆中所有的标本都会活过来,追逐骨头的霸王龙、爱捣乱的猴子、暴虐的匈奴王、钻木取火的穴居人……将一切弄得不可开交。在26任总统特迪·罗斯福的帮助下,达利好不容易控制住了局面,但是,他马上又遇到了新的麻烦:有人想打法老金牌的主意。职责所在,也是为了博物馆夜晚的奇迹,达利不得不挺身而出。In New York, unemployed and divorced Larry Daley is a complete loser. His son Nick is very disappointed with his father who is going to be evicted. Larry accepts the job of night watchman in the Museum of Natural History and takes the place of three old security guards that have just retired in order to raise some money and pay his bills. On his first shift, Larry soon realizes that everything at the museum is not as it seems as the statues begin to come to life after the sun sets. The Museum transforms into complete chaos with the inexperienced Larry in charge as he learns that an old Egyptian stone that came to the Museum in 1950 brings these statues to life until dawn. When Larry brings his son to spend a night with him, the three old guards break into the Museum to try to steal the magical stone. Larry organizes all the historic characters to help him stop the criminals and save the museum.